We Provide

The Best Flow Cytometry
Courses for You

OUR ACCREDITED COURSES
FOR SCYM PREP AND CMLE CREDITS

Supernova Flow Cytometry offers the first comprehensive, accredited training pathway in flow cytometry, delivering unique value on multiple fronts.These courses are designed to be taken sequentially for a complete training experience, but can also be taken à la carte to fill specific knowledge gaps. Each course is fully online (accessible via our learning management system) and also offered periodically as a live virtual class for those who prefer real-time instruction. In addition, we schedule certain courses as in-person workshops for hands-on practice (often bundling related courses into a 3-4 day on-site training session). Upon successful completion of each course, learners receive a certificate and earn continuing education credits (CMLE hours).

 

  • Structured, End-to-End Curriculum: We provide a cohesive 12-course program that covers everything from basic principles to advanced, specialized topics. This far-reaching curriculum is unparalleled – current training options are often fragmented, focusing on either introductory theory or niche applications, but not both. Supernova Flow Cytometry’s program ensures learners build fundamental knowledge (optics, fluidics, data analysis) before progressing to complex topics (multicolor panel design, regulatory compliance, high-dimensional data analysis). This integrated approach helps standardize best practices across research and clinical labs, addressing the current fragmentation in cytometry training.
 
 
  • SuperNova Flow Cytometry offers a fully accredited, peer-reviewed curriculum designed for both clinical and research flow cytometry professionals. All courses provide CMLE credits approved by the American Society of Cytopathology and accepted by ASCP for certification maintenance—an essential benefit for certified lab personnel. Graduates receive certificates and credit hours (13+ for completing all 12 courses), making SuperNova Flow Cytometry a highly efficient option for professional development.

  • The curriculum is also aligned with the SCYM(ASCP) certification exam, incorporating exam-style questions and objectives to prepare trainees for this advanced credential. This dual focus on continuing education and SCYM preparation sets SuperNova Flow Cytometry apart from competitors and positions it as a potential unofficial SCYM prep provider—making it a strategic choice for career-focused cytometrists.

  • Declaration of generative AI and AI-assisted technologies in the writing process: During revision and generation of the courses and other collateral, we  used ChatGPT LLM models (OpenAI) to assist with organization, language editing, comparison of manuscript versions.  We used NanoBanana and OpusAgent to generate AI images and  AI movies to better illustrate concepts. The author and other experts at SuperNova Flow Cytometry independently reviewed, verified, and revised all scientific and regulatory content and take full responsibility for the content in any and all SuperNova Flow Cytometry scientific collateral.

For customized courses, please contact caguilerasandoval@supernova-flowcytometry.com by indicating “Customized Course” in the subject line.







✖ Clear Filters

    Course 1: Introduction to Flow Cytometry and Comparing Conventional v Spectral

    Explore flow cytometer design—optics, fluidics, and detection systems. Learn how lasers, filters, and PMTs work together to generate, focus, and interpret fluorescence signals in research and CGT manufacturing.   ...

    Course 10: CAR-T and Gene Therapy Applications

    Flow cytometry across CGT: CAR detection strategies, gating, T-cell phenotyping, potency & MRD assays, QC/validation (CLSI H62/MIFlowCyt), and spectral vs conventional platforms for research, manufacturing, and clinical monitoring.

    Course 11: FDA Regulatory Requirements for Flow Cytometry

    Explore FDA regulatory expectations for flow cytometry in cell and gene therapy, including assay validation (CLSI H62, ICH Q2), 21 CFR Part 11, CLIA/LDTs, and CAR-T QC applications. Earn 1 ASCP CMLE credit. ...

    Course 12: Flow Cytometry Advanced Analysis

    Learn how high-dimensional flow cytometry propels cell- and gene-therapy programs—from selecting manual, automated, or hybrid gating to extracting hidden cell populations with FlowSOM, PhenoGraph, t-SNE, UMAP, and supervised tools like CITRUS or SVM—while maintaining grade data integrity and audit readiness. You’ll also glimpse the future of digital cytometry, including real-time analytics, multi-omics fusion, and scalable AI pipelines that turn millions of events into actionable, regulatory-grade biomarkers.

    Course 2- Instrumentation – Optics, Fluidics, and Detection

    Explore how lasers, optics, fluidics, and detectors integrate with spectral techniques, voltage tuning, and multicolor panel design to meet real flow assay needs. Complete the brief assessment to earn a CMLE-accredited certificate that confirms your ability to choose, optimize, and troubleshoot flow-cytometry platforms.

    Course 3: Fluorochromes and Compensation

    Design and run high-parameter flow panels with confidence: fluorochrome physics, brightness and Stain Index, spillover and spreading error, compensation matrices and controls, tandem dye pitfalls, and spectral vs conventional detection.

    Course 4: Panel Design and Reagent Selection

    Optimize flow cytometry panels for CGT: reagent prep, titration, FMOs, single-color controls, conventional vs spectral strategies, QC trending, and benchmarking. Practical, defensible methods for academia and industry.

    Course 5: Gating Strategies and Data Analysis

    Master logical gating for CGT: root→lineage→subset gating, back-gating, FMOs, template workflows, bias control, batch normalization, compensation vs spectral unmixing, and QC practices for rare-event, reproducible analyses.

    Course 6: Cell Viability and Apoptosis

    Learn technical principles of flow cytometry-based viability and apoptosis assays, including dye selection, gating, assay optimization, and interpretation for reliable cell health assessment in CGT workflows.

    Course 7: Immunophenotyping and Clinical Diagnostics

    Master clinical immunophenotyping: T/B/NK panels, leukemia/lymphoma screening, standardized gating, QC, and spectral vs conventional flow. Apply best practices for diagnostics and CGT manufacturing.

    Course 8: Cell Sorting and Single-Cell Applications

    Master FACS for CGT: droplet physics, jet-in-air vs cuvette, biosafety/AMS, purity vs yield vs index modes, spectral sorting/unmixing, GMP/closed systems, and post-sort QC for clonal, CRISPR, and single-cell omics workflows.

    Course 9: Assay and Panel Validation for RUO cGMP GLP

    Discover how to build and defend a regulatory-grade validation plan—from RUO to cGMP and IVD—by designing studies for specificity, accuracy, precision, linearity, and LOD/LOQ while aligning with both FDA and CLIA expectations.

    pricing plan 3

    Placeholder

    Pricing Plan2

    Placeholder