– Understand the principles and applications of cell viability and apoptosis assays in flow cytometry.
– Identify and interpret the use of key dyes such as 7-AAD, PI, Zombie dyes, and Annexin V.
– Design and optimize experimental workflows for viability and apoptosis analysis in CGT manufacturing.
– Apply quadrant gating and data analysis to distinguish live, dead, early and late apoptotic cells.
– Recognize the regulatory requirements (FDA, GMP, RUO) for viability and apoptosis testing in cell
therapy.
– Evaluate emerging technologies in flow cytometry such as spectral cytometry and caspase-based
assays.
– Implement best practices from peer-reviewed literature and professional societies (ISAC, ICCS, CLSI).
$9.99
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