End-to-end QC perspective – Learn how flow cytometry verifies identity, purity, viability, and functional potency of CGT products, so your data stand up to regulatory scrutiny.
Fluent gating skills – Move confidently from root to lineage to subset gates, mastering hierarchical strategies that reveal complex immune phenotypes without losing rare events.
Manual + automated mastery – Compare hand-drawn regions with algorithm-based clustering and discover hybrid workflows that speed analysis while preserving biological context.
Back-gating as proof – Use back-gating and other validation tools to confirm population integrity in high-parameter datasets before results reach QA or the clinic.
Platform-specific know-how – Optimize panel design and data interpretation for both conventional and spectral instruments, understanding where unmixing or compensation can trip you up.
Controls that convince auditors – Deploy FMO tubes, blinded gating protocols, and audit-trail documentation to minimize subjectivity and maximize reproducibility.
Software versatility – Navigate FlowJo, FCS Express, Kaluza, and Cytobank, choosing the right platform—and plug-ins—for clinical trials, manufacturing QC, or exploratory research.
Data-quality safeguards – Detect batch effects, tandem-dye decay, and instrument drift early, applying corrective analytics to keep longitudinal studies on track.
Unmixing & metrics – Verify spectral unmixing with chi-square error and related statistics, ensuring high-dimensional panels deliver clean separation.
Design like a pro – Apply brightness matching, spectral-overlap minimization, and smart marker distribution to build panels that scale past 20 colors without sacrificing clarity.
$255.00
