Fluorochrome fluency – Grasp how excitation and emission spectra, brightness, and Stain Index (SI) guide fluorochrome selection for high-parameter single-cell analysis.
Spectral-overlap savvy – Recognize spillover’s impact on data quality and apply practical strategies to minimize it before you even hit “acquire.”
Compensation confidence – Build, troubleshoot, and validate compensation matrices—mastering the math behind spillover coefficients for clean, reliable results.
Panel-design principles – Match fluorochrome characteristics (FITC, PE, APC, Brilliant Violet, PerCP, and more) to instrument lasers and detectors for balanced multicolor panels.
Instrument insight – Compare conventional and spectral cytometers, weighing their advantages for different CGT workflows and sample types.
Quality-control expertise – Account for photobleaching, fixation sensitivity, and spillover spreading to keep signal integrity high from preparation to analysis.
Manual vs. automated workflows – Evaluate software-driven compensation and AI-assisted gating tools to streamline your data-analysis pipeline.
Future-ready perspective – Explore emerging trends such as spectral unmixing algorithms, lifetime cytometry, and machine-learning-powered analytics—so you stay ahead of the curve.
$255.00
