Viability & Apoptosis Essentials – Grasp the scientific principles behind live-/dead discrimination and apoptotic profiling so you can choose the right assay for any CGT workflow.
Fluorochrome Familiarity – Confidently work with 7-AAD, Propidium Iodide, fixable Zombie dyes, and Annexin V, understanding when each dye delivers the clearest signal and lowest background.
Workflow Design Skills – Build and optimize end-to-end protocols—from sample prep to acquisition settings—that keep viability and apoptosis readouts reproducible on the manufacturing floor.
Quadrant-Gating Mastery – Apply precise quadrant gating to distinguish live, dead, early-, and late-apoptotic populations, then translate those numbers into actionable release criteria.
Regulatory Alignment – Navigate FDA and GMP expectations for viability/apoptosis assays, including when RUO reagents are acceptable and how to justify them in filings.
Tech-Forward Perspective – Evaluate spectral cytometry and caspase-based assays so you’re prepared to incorporate next-generation tools as soon as they’re validated.
Evidence-Based Practice – Integrate peer-reviewed guidelines and ISAC/ICCS/CLSI best practices, ensuring your data withstand auditor—and journal—scrutiny.