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The Best Flow Cytometry
Courses for You

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OUR ACCREDITED COURSES
FOR SCYM PREP AND CMLE CREDITS

Supernova Flow Cytometry offers the first comprehensive, accredited training pathway in flow cytometry, delivering unique value on multiple fronts.These courses are designed to be taken sequentially for a complete training experience, but can also be taken à la carte to fill specific knowledge gaps. Each course is fully online (accessible via our learning management system) and also offered periodically as a live virtual class for those who prefer real-time instruction. In addition, we schedule certain courses as in-person workshops for hands-on practice (often bundling related courses into a 3-4 day on-site training session). Upon successful completion of each course, learners receive a certificate and earn continuing education credits (CMLE hours).


  • Structured, End-to-End Curriculum: We provide a cohesive 12-course program that covers everything from basic principles to advanced, specialized topics. This far-reaching curriculum is unparalleled – current training options are often fragmented, focusing on either introductory theory or niche applications, but not both. Supernova’s program ensures learners build fundamental knowledge (optics, fluidics, data analysis) before progressing to complex topics (multicolor panel design, regulatory compliance, high-dimensional data analysis). This integrated approach helps standardize best practices across research and clinical labs, addressing the current fragmentation in cytometry training.

  • SuperNova offers a fully accredited, peer-reviewed curriculum designed for both clinical and research flow cytometry professionals. All courses provide CMLE credits approved by the American Society of Cytopathology and accepted by ASCP for certification maintenance—an essential benefit for certified lab personnel. Graduates receive certificates and credit hours (13+ for completing all 12 courses), making SuperNova a highly efficient option for professional development.

  • The curriculum is also aligned with the SCYM(ASCP) certification exam, incorporating exam-style questions and objectives to prepare trainees for this advanced credential. This dual focus on continuing education and SCYM preparation sets SuperNova apart from competitors and positions it as a potential unofficial SCYM prep provider—making it a strategic choice for career-focused cytometrists.

For customized courses, please contact caguilerasandoval@supernova-flowcytometry.com by indicating “Customized Course” in the subject line.




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    Course 1 - Introduction to Flow Cytometry (Conventional vs. Spectral)

    Topics Covered:
    • Basic principles: hydrodynamic focusing, fluorescence, scatter
    • Applications in research, diagnostics, and therapeutics
    • Overview of key components: lasers, detectors, filters
    • Instrument safety and biosafety level considerations
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    Course 10: CAR-T and Gene Therapy Applications

    Master how conventional and spectral flow cytometry quantify CAR-T identity, purity, potency, and cytotoxicity—from CLSI H62-aligned validation and regulatory-grade gating to minimal-residual-disease detection. You’ll connect immunophenotyping markers to clinical outcomes, generate FDA-ready IND/BLA datasets under MIFlowCyt and CLIA/CAP rules, and troubleshoot technical pitfalls for reliable immunomonitoring across trials.
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    Course 11: FDA Regulatory Requirements for Flow Cytometry

    Learn how flow cytometry moves from bench to bedside under FDA oversight—mastering 21 CFR Part 11, CLIA, GLP, and cGMP rules—while designing IND/BLA-ready assays that meet ICH Q2(R1) validation, CDx/MRD expectations, and strict data-integrity standards for potency, identity, purity, and safety testing in cell- and gene-therapy products.

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    Course 12: Flow Cytometry Advanced Analysis

    Learn how high-dimensional flow cytometry propels cell- and gene-therapy programs—from selecting manual, automated, or hybrid gating to extracting hidden cell populations with FlowSOM, PhenoGraph, t-SNE, UMAP, and supervised tools like CITRUS or SVM—while maintaining grade data integrity and audit readiness. You’ll also glimpse the future of digital cytometry, including real-time analytics, multi-omics fusion, and scalable AI pipelines that turn millions of events into actionable, regulatory-grade biomarkers.
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    Course 2- Instrumentation – Optics, Fluidics, and Detection

    Explore how lasers, optics, fluidics, and detectors integrate with spectral techniques, voltage tuning, and multicolor panel design to meet real flow assay needs. Complete the brief assessment to earn a CMLE-accredited certificate that confirms your ability to choose, optimize, and troubleshoot flow-cytometry platforms.
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    Course 3: Fluorochromes and Compensation

    Dive into the physics that drives fluorochrome choice—excitation / emission spectra, brightness, spillover, and compensation math—then compare conventional versus spectral cytometers, tackle QC pitfalls like photobleaching, and preview next-gen tools such as AI-assisted gating and lifetime cytometry. In short, you’ll master every step from panel design to data integrity while seeing where the field is headed.
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    Course 4: Panel Design and Reagent Selection

    Gain the skills to design, validate, and troubleshoot high-dimensional flow cytometry panels that power identity, purity, viability, and potency assays in CGT manufacturing and trials—while aligning every reagent, control, and metric with FDA expectations. By course end you’ll be fluent in both conventional and spectral strategies, ready to tackle spectral spillover and tandem-dye drift, and positioned to excel on the Flow Cytometry Certification Exam.
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    Course 5: Gating Strategies and Data Analysis

    Learn the full data-analysis pipeline for flow cytometry—from hierarchical gating (root → lineage → subset) and back-gating validation to software-driven, hybrid manual/automated workflows—while learning to design high-dimensional panels, verify spectral unmixing, and control batch effects, drift, and subjectivity with FMO and audit-trail safeguards. By the end, you’ll know how to translate complex immune phenotypes into reproducible identity, purity, viability, and function readouts that meet regulatory expectations.
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    Course 6: Cell Viability and Apoptosis

    Learn how to build CGT-grade viability and apoptosis assays—from selecting 7-AAD, PI, Zombie dyes, and Annexin V to applying quadrant gating that cleanly separates live, dead, and early-/late-apoptotic cells. You’ll also see how FDA/GMP expectations, spectral cytometry, and caspase-based readouts shape the next wave of best-practice workflows.
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    Course 7: Immunophenotyping and Clinical Diagnostics

    Master the essentials of flow cytometry—from core physics to high-dimensional spectral analysis—while learning to immunophenotype T, B, and NK cells in health and in leukemias such as T-ALL, B-ALL, and AML. You’ll design standardized panels, apply reproducible gating, and meet CLIA/CAP/FDA requirements so your data drive confident clinical diagnoses and CGT product release.
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    Course 8: Cell Sorting and Single-Cell Applications

    Learn how to choose and optimize droplet-based or spectral cell sorters—balancing purity, yield, and index modes—while meeting GMP biosafety and regulatory demands. You’ll compare cuvette vs. jet-in-air systems, master aerosol containment, and translate high-quality sorts into scRNA-seq, CAR-T, and CRISPR workflows without sacrificing cell health.
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    Course 9: Assay and Panel Validation for RUO cGMP GLP CLIA and IVD

    Discover how to build and defend a regulatory-grade validation plan—from RUO to cGMP and IVD—by designing studies for specificity, accuracy, precision, linearity, and LOD/LOQ while aligning with both FDA and CLIA expectations. You’ll learn to document robustness, carryover, and spike-in recovery tests; validate CAR-T identity and potency panels with proper controls and gating; and apply CLSI H62/MIFlowCyt guidance to conventional and spectral platforms.
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Get in Touch

If you have any questions, then please feel free to drop me a line at caguilerasandoval@supernova-flowcytometry.com

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