QC-ready insight – See exactly how flow cytometry underpins identity, purity, viability, and potency assays for CGT manufacturing, lot release, and clinical trials.
Panel-design fluency – Match fluorochromes to antigen density, evaluate co-expression patterns, and minimize spectral overlap so your data are publication- and submission-ready.
Titration that delivers – Master antibody titration and validation on real manufacturing samples to maximize stain index and day-to-day reproducibility.
Control strategy know-how – Deploy FMO, single-color, viability, and CAR-T reference controls to tighten gates and boost confidence in every decision point.
Regulatory alignment – Select RUO, IVD, or GMP-grade reagents wisely and justify them in FDA-regulated assays, IND submissions, and multi-site harmonization studies.
Conventional vs. spectral skills – Compare instrument platforms and learn design tactics that exploit each system’s strengths while avoiding common pitfalls.
High-dimensional optimization – Use Stain Index, Complexity Index, Similarity Index, and Spillover-Spread Matrix to refine panels that push past 20 colors with clean separation.
Troubleshooting toolkit – Spot and fix spectral spillover, autofluorescence, tandem-dye drift, and unmixing errors before they tank your run.
Certification momentum – Build the foundational expertise that positions you for the Flow Cytometry Certification Exam and for impactful work on regulated CGT assays.
