Topics Covered:
– Basic principles: hydrodynamic focusing, fluorescence, scatter
– Applications in research, diagnostics, and therapeutics
– Overview of key components: lasers, detectors, filters
– Instrument safety and biosafety level considerations
What you’ll take away from the introductory course:
Understand the essentials – Get a clear picture of why flow cytometry is critical in cell- and gene-therapy workflows and where it fits in routine QC.
Know the instrument basics – Recognize the role of the core hardware elements (lasers, optics, detectors, fluidics) and what each contributes to data quality.
Conventional vs. spectral—at a glance – Learn the practical differences between filter-based and full-spectrum systems so you can select the right platform for straightforward assays.
Sound sample prep and starter panel design – Follow step-by-step guidance on handling cells, choosing fluorochromes, and assembling a small (3–4 color) panel that minimizes beginner pitfalls.
Introductory data analysis skills – Walk through a simple gating and unmixing exercise with example files to see how populations are identified and common artifacts spotted.
Verified learning – Complete a short quiz; passing earns a certificate of completion with CMLE credit hours you can apply toward ASCP continuing-education requirements.