Course 6: Cell Viability and Apoptosis

What You’ll Gain from This Course

  • Viability & Apoptosis Essentials – Grasp the scientific principles behind live-/dead discrimination and apoptotic profiling so you can choose the right assay for any CGT workflow.

  • Fluorochrome Familiarity – Confidently work with 7-AAD, Propidium Iodide, fixable Zombie dyes, and Annexin V, understanding when each dye delivers the clearest signal and lowest background.

  • Workflow Design Skills – Build and optimize end-to-end protocols—from sample prep to acquisition settings—that keep viability and apoptosis readouts reproducible on the manufacturing floor.

  • Quadrant-Gating Mastery – Apply precise quadrant gating to distinguish live, dead, early-, and late-apoptotic populations, then translate those numbers into actionable release criteria.

  • Regulatory Alignment – Navigate FDA and GMP expectations for viability/apoptosis assays, including when RUO reagents are acceptable and how to justify them in filings.

  • Tech-Forward Perspective – Evaluate spectral cytometry and caspase-based assays so you’re prepared to incorporate next-generation tools as soon as they’re validated.

  • Evidence-Based Practice – Integrate peer-reviewed guidelines and ISAC/ICCS/CLSI best practices, ensuring your data withstand auditor—and journal—scrutiny.

 

SKU course-86 Category

$255.00